ORCA-Flash4.0 V3 Digital CMOS camera C13440-20CU
Building on our extensive experience with high performance scientific cameras and advanced imaging applications, Hamamatsu introduces the new ORCA-Flash4.0 V3. This one camera expertly handles applications ranging from the acquisition of beautiful scientific images to experiments that demand detection, quantification and speed. With on-board FPGA processing enabling intelligent data reduction, highly refined in-camera, pixel-level calibrations, increased USB3.0 frame rates, purposeful and innovative triggering capabilities, patented lightsheet read out modes and individual camera noise characterization the ORCA-Flash4.0 V3 is the precision instrument for imaging.
Special sites
The life science camera section is the feature section for digital cameras fit to life science research and industry areas.
Features
- Calibrated for Quantitative Accuracy
- Flexibility for Customized Data Control
- Patented Tools for Advanced Imaging: Lightsheet Readout Mode
- Focus on the Relevant Data
- Powerful Triggering for Synchronizationa
Movie gallery
3D animation of Amoeba scanned by DSLM
GFP-labeled zebrafish blood flow
High speed (400 fps) zebrafish cardiac beat
iPS cardiomyocyte membrane voltage dynamics (high resolution)
Localization microscopy acquisition
Dynamic movement of shrimp swimmeret
Spinning disk confocal pollen z scan images
Filopodial protrusion dynamics
High-speed intracellular Ca gradient driven by UTP stimulation
Microtubule growth dynamics TIRF
PKC translocation driven by TEA stimulation
High-speed Ca imaging of iPS cardiomyocyte
PC recommendations
With the introduction of the ORCA-Flash4.0, users are now able to stream 4 megapixel images to their computers 100 frames per second. The computer recommendations for this high data rate can be met by using the guidelines listed this PC Recommendations for ORCA-Flash4.0.
Specifications
| Type number | C13440-20CU |
|---|---|
| Quantum efficiency | 82 % (peak QE: @560 nm) |
| Imaging device | sCMOS |
| Effective no. of pixels | 2048 (H)×2048 (V) |
| Cell size | 6.5 μm×6.5 μm |
| Effective area | 13.312 mm×13.312 mm |
| Full well capacity | 30 000 electrons (typ.) * |
| Readout speed | 100 frames/s (Full resolution, standard scan, Camera Link) * 40 frames/s (Full resolution, standard scan, USB 3.0, 16 bit) * 53 frames/s (Full resolution, standard scan, USB 3.0, 12 bit) * 80 frames/s (Full resolution, standard scan, USB 3.0, 8 bit) * |
| Readout noise | Standard scan (at 100 frames/s, typ.):1.6 electrons rms (1.0 electrons median) Slow scan (at 30 frames/s, typ.): 1.4 electrons rms (0.8 electrons median) |
| Cooling method | Peltier cooling |
| Cooling temperature | Forced air (Ambient at +20 ℃): -10 ℃ Water (+20 ℃): -10 ℃ Water (+15 ℃): -30 ℃ |
| Dark current | 0.06 electrons/pixel/s (Air Cooled to -10° C) (typ.) 0.06 electrons/pixel/s (Water Cooled to -10° C) (typ.) 0.006 electrons/pixel/s (Water Cooled to -30° C) (typ.) |
| Dynamic range | 37 000:1 (typ.) |
| Interface | Camera Link / USB 3.0 |
| A/D converter | 16 bit / 12 bit / 8 bit |
| Lens mount | C-mount |
* Typical value 2 Enabled with optional Camera Link board for PC
| Readout Modes | Normal Area, Lightsheet, W-VIEW Mode, Dual Lightsheet |
|---|---|
| Binning | 2×2, 4×4 |
| Master Pulse Generator (Pulse Modes) | Internal Sync, Start Trigger, Burst |
| Master Pulse Generator (Pulse Interval in 1 μs increments) | 10 μs to 10 s |
| Hot Pixel Correction | Off, Low, Medium, High |
| Dark Signal Non-Uniformity (DSNU)* | 0.3 electrons rms |
| Photo Response Non-Uniformity (PRNU) at half level of full light range (15,000 electrons)* | 0.06 % rms |
| Photo Response Non-Uniformity (PRNU) at low light level (700 electrons)* | 0.3 % rms |
| Linearity error, full light range (EMVA 1288 standard)* | 0.5 % |
| Linearity error, low light range (< 500 electrons signal)* | 0.2 % / Less than approx. 1 electron absolute error |
| On-camera Connectivity | Both USB 3.0 and Camera Link** |
| V2 Compatibility Mode (for use with legacy software) | Yes |
*Typical value
**Enabled with optional Camera Link board for PC
Spectral response
Dimensions
