ORCA-Flash4.0 V3 Digital CMOS camera C13440-20CU

c13440-20cu product photo — **Catalog (For lifescience) [939 KB/PDF]**

Building on our extensive experience with high performance scientific cameras and advanced imaging applications, Hamamatsu introduces the new ORCA-Flash4.0 V3. This one camera expertly handles applications ranging from the acquisition of beautiful scientific images to experiments that demand detection, quantification and speed. With on-board FPGA processing enabling intelligent data reduction, highly refined in-camera, pixel-level calibrations, increased USB3.0 frame rates, purposeful and innovative triggering capabilities, patented lightsheet read out modes and individual camera noise characterization the ORCA-Flash4.0 V3 is the precision instrument for imaging.

Features

Calibrated for Quantitative Accuracy
Flexibility for Customized Data Control
Patented Tools for Advanced Imaging
Focus on the Relevant Data
Powerful Triggering for Synchronizationa

Movie gallery

3D animation of Amoeba scanned by DSLM

GFP-labeled zebrafish blood flow

High speed (400 fps) zebrafish cardiac beat

iPS cardiomyocyte membrane voltage dynamics (high resolution)

Localization microscopy acquisition

Dynamic movement of shrimp swimmeret

Spinning disk confocal pollen z scan images

Filopodial protrusion dynamics

High-speed intracellular Ca gradient driven by UTP stimulation

Microtubule growth dynamics TIRF

PKC translocation driven by TEA stimulation

High-speed Ca imaging of iPS cardiomyocyte

PC requirements

With the introduction of the ORCA-Flash4.0, users are now able to stream 4 megapixel images to their computers 100 frames per second. The computer requirements for this high data rate can be met by using the guidelines listed this PC Recommendations for ORCA-Flash4.0.

PC Recommendations for ORCA-Flash4.0 V3/LT+ [764 KB/PDF]

Specifications

Type number	C13440-20CU
Quantum efficency	82 % (peak QE: @560 nm)
Imaging device	sCMOS
Effective no. of pixels	2048 (H)×2048 (V)
Cell size	6.5 μm×6.5 μm
Effective area	13.312 mm×13.312 mm
Full well capacity	30 000 electrons (typ.) *
Readout speed	100 frames/s (Full resolution, standard scan, Camera Link) * 40 frames/s (Full resolution, standard scan, USB 3.0, 16 bit) * 53 frames/s (Full resolution, standard scan, USB 3.0, 12 bit) * 80 frames/s (Full resolution, standard scan, USB 3.0, 8 bit) *
Readout noise	Standard scan (at 100 frames/s, typ.):1.6 electrons rms (1.0 electrons median) Slow scan (at 30 frames/s, typ.): 1.4 electrons rms (0.8 electrons median)
Cooling method	Peltier cooling
Cooling temperature	Forced air (Ambient at +20 ℃): -10 ℃ Water (+20 ℃): -10 ℃ Water (+15 ℃): -30 ℃
Dark current	0.06 electrons/pixel/s (Air Cooled to -10° C) (typ.) 0.06 electrons/pixel/s (Water Cooled to -10° C) (typ.) 0.006 electrons/pixel/s (Water Cooled to -30° C) (typ.)
Dynamic range	37 000:1 (typ.)
Interface	Camera Link / USB 3.0
A/D converter	16 bit / 12 bit / 8 bit
Lens mount	C-mount

Readout Modes	Normal Area, Lightsheet, W-VIEW Mode, Dual Lightsheet
Binning	2×2, 4×4
Master Pulse Generator (Pulse Modes)	Internal Sync, Start Trigger, Burst
Master Pulse Generator (Pulse Interval in 1 μs increments)	10 μs to 10 s
Hot Pixel Correction	Off, Low, Medium, High
Dark Signal Non-Uniformity (DSNU)*	0.3 electrons rms
Photo Response Non-Uniformity (PRNU) at half level of full light range (15,000 electrons)*	0.06 % rms
Photo Response Non-Uniformity (PRNU) at low light level (700 electrons)*	0.3 % rms
Linearity error, full light range (EMVA 1288 standard)*	0.5 %
Linearity error, low light range (< 500 electrons signal)*	0.2 % / Less than approx. 1 electron absolute error
On-camera Connectivity	Both USB 3.0 and Camera Link**
V2 Compatibility Mode (for use with legacy software)	Yes