ORCA-Flash4.0 V3 Digital CMOS camera C13440-20CU
Building on our extensive experience with high performance scientific cameras and advanced imaging applications, Hamamatsu introduces the new ORCA-Flash4.0 V3. This one camera expertly handles applications ranging from the acquisition of beautiful scientific images to experiments that demand detection, quantification and speed. With on-board FPGA processing enabling intelligent data reduction, highly refined in-camera, pixel-level calibrations, increased USB3.0 frame rates, purposeful and innovative triggering capabilities, patented lightsheet read out modes and individual camera noise characterization the ORCA-Flash4.0 V3 is the precision instrument for imaging.
Special sites
The scientific camera section is the feature section for digital cameras fit to life science research and industry areas.
Features
- Calibrated for Quantitative Accuracy
- Flexibility for Customized Data Control
- Patented Tools for Advanced Imaging: Lightsheet Readout Mode
- Focus on the Relevant Data
- Powerful Triggering for Synchronizationa
Lightsheet Readout Mode [Patented]
“Lightsheet Readout Mode” is a unique and patented feature of Hamamatsu sCMOS cameras which can improve signal to noise ratios in Lightsheet microscopy.
For more information about the principle and features of Lightsheet Readout Mode, please see details from below.
Movie gallery
3D animation of Amoeba scanned by DSLM
GFP-labeled zebrafish blood flow
High speed (400 fps) zebrafish cardiac beat
iPS cardiomyocyte membrane voltage dynamics (high resolution)
Localization microscopy acquisition
Spinning disk confocal pollen z scan images
Filopodial protrusion dynamics
High-speed intracellular Ca gradient driven by UTP stimulation
Microtubule growth dynamics TIRF
PKC translocation driven by TEA stimulation
High-speed Ca imaging of iPS cardiomyocyte
PC recommendations
With the introduction of the ORCA-Flash4.0, users are now able to stream 4 megapixel images to their computers 100 frames per second. The computer recommendations for this high data rate can be met by using the guidelines listed this PC Recommendations for ORCA-Flash4.0.
Specifications
| Type number | C13440-20CU |
|---|---|
| Quantum efficiency | 82 % (peak QE: @560 nm) |
| Imaging device | sCMOS |
| Effective no. of pixels | 2048 (H)×2048 (V) |
| Cell size | 6.5 μm×6.5 μm |
| Effective area | 13.312 mm×13.312 mm |
| Full well capacity | 30 000 electrons (typ.) * |
| Readout speed | 100 frames/s (Full resolution, standard scan, Camera Link) * 40 frames/s (Full resolution, standard scan, USB 3.0, 16 bit) * 53 frames/s (Full resolution, standard scan, USB 3.0, 12 bit) * 80 frames/s (Full resolution, standard scan, USB 3.0, 8 bit) * |
| Readout noise | Standard scan (at 100 frames/s, typ.):1.6 electrons rms (1.0 electrons median) Slow scan (at 30 frames/s, typ.): 1.4 electrons rms (0.8 electrons median) |
| Cooling method | Peltier cooling |
| Cooling temperature | Forced air (Ambient at +20 ℃): -10 ℃ Water (+20 ℃): -10 ℃ Water (+15 ℃): -30 ℃ |
| Dark current | 0.06 electrons/pixel/s (Air Cooled to -10° C) (typ.) 0.06 electrons/pixel/s (Water Cooled to -10° C) (typ.) 0.006 electrons/pixel/s (Water Cooled to -30° C) (typ.) |
| Dynamic range | 37 000:1 (typ.) |
| Interface | Camera Link / USB 3.0 |
| A/D converter | 16 bit / 12 bit / 8 bit |
| Lens mount | C-mount |
* Typical value 2 Enabled with optional Camera Link board for PC
| Readout Modes | Normal Area, Lightsheet, W-VIEW Mode, Dual Lightsheet |
|---|---|
| Binning | 2×2, 4×4 |
| Master Pulse Generator (Pulse Modes) | Internal Sync, Start Trigger, Burst |
| Master Pulse Generator (Pulse Interval in 1 μs increments) | 10 μs to 10 s |
| Hot Pixel Correction | Off, Low, Medium, High |
| Dark Signal Non-Uniformity (DSNU)* | 0.3 electrons rms |
| Photo Response Non-Uniformity (PRNU) at half level of full light range (15,000 electrons)* | 0.06 % rms |
| Photo Response Non-Uniformity (PRNU) at low light level (700 electrons)* | 0.3 % rms |
| Linearity error, full light range (EMVA 1288 standard)* | 0.5 % |
| Linearity error, low light range (< 500 electrons signal)* | 0.2 % / Less than approx. 1 electron absolute error |
| On-camera Connectivity | Both USB 3.0 and Camera Link** |
| V2 Compatibility Mode (for use with legacy software) | Yes |
*Typical value
**Enabled with optional Camera Link board for PC
Spectral response
Dimensions
