ORCA-Flash4.0 V3 Digital CMOS camera C13440-20CU

c13440-20cu product photo — **Catalog (For lifescience) [939 KB/PDF]**

Building on our extensive experience with high performance scientific cameras and advanced imaging applications, Hamamatsu introduces the new ORCA-Flash4.0 V3. This one camera expertly handles applications ranging from the acquisition of beautiful scientific images to experiments that demand detection, quantification and speed. With on-board FPGA processing enabling intelligent data reduction, highly refined in-camera, pixel-level calibrations, increased USB3.0 frame rates, purposeful and innovative triggering capabilities, patented lightsheet read out modes and individual camera noise characterization the ORCA-Flash4.0 V3 is the precision instrument for imaging.

Features

Calibrated for Quantitative Accuracy
Flexibility for Customized Data Control
Patented Tools for Advanced Imaging: Lightsheet Readout Mode
Focus on the Relevant Data
Powerful Triggering for Synchronizationa

Movie gallery

3D animation of Amoeba scanned by DSLM

GFP-labeled zebrafish blood flow

High speed (400 fps) zebrafish cardiac beat

iPS cardiomyocyte membrane voltage dynamics (high resolution)

Localization microscopy acquisition

Dynamic movement of shrimp swimmeret

Spinning disk confocal pollen z scan images

Filopodial protrusion dynamics

High-speed intracellular Ca gradient driven by UTP stimulation

Microtubule growth dynamics TIRF

PKC translocation driven by TEA stimulation

High-speed Ca imaging of iPS cardiomyocyte

PC recommendations

With the introduction of the ORCA-Flash4.0, users are now able to stream 4 megapixel images to their computers 100 frames per second. The computer recommendations for this high data rate can be met by using the guidelines listed this PC Recommendations for ORCA-Flash4.0.

PC Recommendations for ORCA-Flash4.0 V3/LT+ [764 KB/PDF]

Specifications

Type number	C13440-20CU
Quantum efficency	82 % (peak QE: @560 nm)
Imaging device	sCMOS
Effective no. of pixels	2048 (H)×2048 (V)
Cell size	6.5 μm×6.5 μm
Effective area	13.312 mm×13.312 mm
Full well capacity	30 000 electrons (typ.) *
Readout speed	100 frames/s (Full resolution, standard scan, Camera Link) * 40 frames/s (Full resolution, standard scan, USB 3.0, 16 bit) * 53 frames/s (Full resolution, standard scan, USB 3.0, 12 bit) * 80 frames/s (Full resolution, standard scan, USB 3.0, 8 bit) *
Readout noise	Standard scan (at 100 frames/s, typ.):1.6 electrons rms (1.0 electrons median) Slow scan (at 30 frames/s, typ.): 1.4 electrons rms (0.8 electrons median)
Cooling method	Peltier cooling
Cooling temperature	Forced air (Ambient at +20 ℃): -10 ℃ Water (+20 ℃): -10 ℃ Water (+15 ℃): -30 ℃
Dark current	0.06 electrons/pixel/s (Air Cooled to -10° C) (typ.) 0.06 electrons/pixel/s (Water Cooled to -10° C) (typ.) 0.006 electrons/pixel/s (Water Cooled to -30° C) (typ.)
Dynamic range	37 000:1 (typ.)
Interface	Camera Link / USB 3.0
A/D converter	16 bit / 12 bit / 8 bit
Lens mount	C-mount

Readout Modes	Normal Area, Lightsheet, W-VIEW Mode, Dual Lightsheet
Binning	2×2, 4×4
Master Pulse Generator (Pulse Modes)	Internal Sync, Start Trigger, Burst
Master Pulse Generator (Pulse Interval in 1 μs increments)	10 μs to 10 s
Hot Pixel Correction	Off, Low, Medium, High
Dark Signal Non-Uniformity (DSNU)*	0.3 electrons rms
Photo Response Non-Uniformity (PRNU) at half level of full light range (15,000 electrons)*	0.06 % rms
Photo Response Non-Uniformity (PRNU) at low light level (700 electrons)*	0.3 % rms
Linearity error, full light range (EMVA 1288 standard)*	0.5 %
Linearity error, low light range (< 500 electrons signal)*	0.2 % / Less than approx. 1 electron absolute error
On-camera Connectivity	Both USB 3.0 and Camera Link**
V2 Compatibility Mode (for use with legacy software)	Yes