ORCA-Fusion BT Digital CMOS camera C15440-20UP

See what you’ve been missing

The ORCA-Fusion BT camera is the pinnacle of scientific CMOS (sCMOS) performance. The specifications are without compromise: ultra-low readout noise, CCD-like uniformity, fast frame rates and back-thinned enabled high QE. But the beauty of the ORCA-Fusion BT is what this combination of exceptional photon detection and collection can do for you. See the dimmest whisper of signal, acquire visually stunning, high S/N images from the fewest photons, capture previously unresolved temporal events and perform computational methods with confidence. The ORCA-Fusion BT is the camera that makes the difficult experiments easy and previously impossible experiments possible.

ORCA is a registered trademark of Hamamatsu Photonics K.K. (China, EU, France, Germany, Japan, UK, USA).

Catalog (For lifescience) 4.8 MB/PDF

Technical note 989 KB/PDF

ORCA-Fusion BT advantages versus other BT CMOS cameras
ORCA-Fusion BT advantages versus EM-CCD cameras
Applications
Lightsheet Readout Mode [Patented]
Publications
PC recommendation
Software
Specifications
Spectral response
Dimensions
Instruction manual

ORCA-Fusion BT advantages versus other BT CMOS cameras

Exclusive industry leading BT sCMOS performance with trusted Hamamatsu quality and reliability

Extremely low readout noise
High QE, across a wide range of wavelengths
CCD-like uniformity; minimal high readout noise pixels
High dynamic range
3 readout speeds
Lightsheet readout mode
5.3 Megapixels
Both USB 3.0 and high speed CoaXPress interfaces

ORCA-Fusion BT advantages versus EM-CCD cameras

A practical and functional replacement for expensive EM-CCDs with uncompromising sensitivity, speed and versatility

Resolution
Binning versatility
No amplification or excess noise
High dynamic range
Large field of view

Applications

Wide-field fluorescence microscopy
Time-lapse imaging
Spinning disk confocal microscopy
Lightsheet microscopy
Super resolution precision localization
Genetically encoded voltage imaging
Optogenetics
Single molecule tracking
Computational imaging
Quantum computing

Time-lapse live cell imaging by low light excitation

Sample: H9c2, Objective lens: Apo TIRF 60XC (NA1.49), ND: 8, Interval: 15 sec

Nucleus

c15440-20up Application example 1

Nucleus:
HCS NuclearMask Stains / Exposure 20 ms

Mitochondria

c15440-20up Application example 3

Mitochondria:
MitoTracker / Exposure 500 ms

Membrane

c15440-20up Application example 2

Membrane:
CellMask Plasma Membrane stains / Exposure 500 ms

Lightsheet Readout Mode [Patented]

“Lightsheet Readout Mode” is a unique and patented feature of Hamamatsu sCMOS cameras which can improve signal to noise ratios in Lightsheet microscopy.
For more information about the principle and features of Lightsheet Readout Mode, please see details from below.

What is Lightsheet Readout Mode?

Publications

ORCA-Fusion BT

Authors	Title	Source
Pratish Thakore, Michael G Alvarado, Sher Ali, Amreen Mughal, Paulo W Pires, Evan Yamasaki, Harry AT Pritchard, Brant E Isakson, Cam Ha T Tran, Scott Earley	Brain endothelial cell TRPA1 channels initiate neurovascular coupling	eLife 10, e63040 (2021).
Lexy von Diezmann and Ofer Rog	Single-Molecule Tracking of Chromatin-Associated Proteins in the C. elegans Gonad	J. Phys. Chem. B 2021, 125, 23, 6162–6170
Kei Yamamoto, Haruko Miura, Motohiko Ishida, Yusuke Mii, Noriyuki Kinoshita, Shinji Takada, Naoto Ueno, Satoshi Sawai, Yohei Kondo & Kazuhiro Aoki	Optogenetic relaxation of actomyosin contractility uncovers mechanistic roles of cortical tension during cytokinesis	Nat Commun 12, 7145 (2021).
Mbaye Diouf, Mitchell Harling, Murat Yessenov, Layton A. Hall, Ayman F. Abouraddy, and Kimani C. Toussaint,	Space-time vector light sheets	Opt. Express 29, 37225-37233 (2021)
Oliver Klatt, Daniele Repetto, Johannes Brockhaus, Carsten Reissner, Abderazzaq El khallouqi, Astrid Rohlmann, Martin Heine, Markus Missler,	Endogenous β-neurexins on axons and within synapses show regulated dynamic behavior	Cell Reports, Volume 35, Issue 11, 2021, 109266,
Satoshi Okada, Goro Doi, Shitomi Nakagawa, Emiko Kusumoto, Takashi Ito	Simple-to-use CRISPR-SpCas9/SaCas9/AsCas12a vector series for genome editing in Saccharomyces cerevisiae	G3 Genes\|Genomes\|Genetics, Volume 11, Issue 12, December 2021, jkab304

PC recommendation

With the introduction of the ORCA-Fusion BT, users are now able to stream 5 megapixel images to their computers 89 frames per second. The computer recommendations for this high data rate can be met by using the guidelines listed this PC Recommendations for ORCA-Fusion BT.

PC Recommendation for ORCA-Fire / Quest2 / FusionBT / Fusion.pdf [588 KB/PDF]

Software

Our software provides the interface to access all of our carefully engineered camera features, from simply setting exposure to orchestrating complex triggering for multidimensional experiments.

Specifications

Product number	C15440-20UP
Imaging device	Scientific CMOS image sensor
Effective number of pixels	2304 (H) × 2304 (V)
Pixel Size	6.5 μm × 6.5 μm
Effective Area	14.976 mm (H) × 14.976 mm (V)
Full well capacity	15 000 electrons^*1
Readout speed	Fast scan, Full resolution, CoaXPress : 89.1 frames/s Fast scan, Full resolution, USB 3.0 : 31.6 frames/s（16 bit）, 42.2 frames/s（12 bit） , 63.3 frames/s（8 bit） Standard scan, Full resolution, CoaXPress : 23.2 frames/s Standard scan, Full resolution, USB 3.0 : 23.2 frames/s Ultra quiet scan, Full resolution, CoaXPress : 5.42 frames/s Ultra quiet scan, Full resolution, USB 3.0 : 5.42 frames/s
Readout noise	Fast scan : 1.6 electrons rms^1 Standard scan : 1.0 electrons rms^1 Ultra-quiet scan: 0.7 electrons rms^*1
Exposure times	Fast scan : 17 µs to 10 s Standard scan : 65 µs to 10 s Ultra quiet scan : 280 µs to 10 s
Cooling method	Peltier cooling
Cooling temperature	Forced-air cooled (Ambient temperature: +25 ˚C) : −8 ˚C Water cooled (Water temperature: +25 ˚C) : −8 ˚C Water cooled (Max cooling (Ambient temperature: +20 ˚C, water temperature: +20 ˚C)) : Less than −15 ˚C
Dark current	1.0 electrons/pixel/s (cooling temperature: -8 ˚C , Ambient temperature: +25 ˚C)^13 1.0 electrons/pixel/s (cooling temperature: -8 ˚C , Water temperature: +25 ˚C)^13 0.7 electrons/pixel/s (cooling temperature: Less than −15 ˚C , Ambient temperature: +20 ˚C, water temperature: +20 ˚C)^13
Dynamic range	21 400:1^12*4
External trigger mode	Edge, Level, Sync readout, Start, Global reset edge, Global reset level, Programmable
External trigger signal routing	SMA
External trigger delay function	0 μs to 10 s (1 μs step)
Trigger output	Global exposure timing, Trigger ready, Low, High, 3 programmable timing outputs
External signal output routing	SMA
Interface	CoaXPress (Dual CXP-6) and USB 3.0 Super Speed^*5
A/D converter	16 bit, 12 bit, 8 bit
Lens mount	C-mount (standard), F-mount (C15440-20UP01)
Power supply	AC 100 V to AC 240 V, 50 Hz/60 Hz
Power consumption	Approx. 155 VA
Ambient operating temperature	0 °C to +40 °C
Ambient storage temperature	-10 ℃ to +50 ℃
Ambient operating humidity	30 % to 80 % (With no condensation)
Ambient storage humidity	90 % or less (With no condensation)