ORCA-Fusion Digital CMOS camera C14440-20UP

The ORCA-Fusion, built from the sensor up, balances the complex nuances of camera features to provide beautiful images and robust data at all lights levels, but especially in tough low-light conditions. The exceptionally low and highly uniform read noise of the ORCA-Fusion means that when the sample emits even just a handful of photons, either by default or by experimental design, they are not lost in the noise, but detected and reliably quantified. After all, when you want to hear a whisper it’s best to be in a quiet place.

ORCA is a registered trademark of Hamamatsu Photonics K.K. (China, EU, Japan, UK, USA).

Catalog (For lifescience) 2.1 MB/PDF

Technical note 989 KB/PDF

Features
Lightsheet Readout Mode [Patented]
Publications
PC recommendations
Software
Specifications
Spectral response
Dimensions
Instruction manual

Features

High resolution : 2304 × 2304 (5.3 Megapixels)
High speed : 100 frames/s (at 2304 × 2048 ROI)
Dynamic range : 21,400:1
Pixel size : 6.5 μm × 6.5 μm
Read noise : 0.7 electrons, rms (Ultra quiet Scan)
Prnu : 0.06 % (@ 7500 electrons)
DSNU : 0.06 electrons
Peak QE : 80 %
Patented Tools for Advanced Imaging: Lightsheet Readout Mode

Lightsheet Readout Mode [Patented]

“Lightsheet Readout Mode” is a unique and patented feature of Hamamatsu sCMOS cameras which can improve signal to noise ratios in Lightsheet microscopy.
For more information about the principle and features of Lightsheet Readout Mode, please see details from below.

What is Lightsheet Readout Mode?

Publications

ORCA-Fusion

Authors	Title	Source
Piatkevich, K.D., Bensussen, S., Tseng, Ha. et al.	Population imaging of neural activity in awake behaving mice	Nature 574, 413–417 (2019).
Silvia Benito-Kwiecinski, Stefano L. Giandomenico, Magdalena Sutcliffe, Erlend S. Riis, Paula Freire-Pritchett, Iva Kelava, Stephanie Wunderlich, Ulrich Martin, Gregory A. Wray, Kate McDole, Madeline A. Lancaster	An early cell shape transition drives evolutionary expansion of the human forebrain	Cell, Volume 184, Issue 8, 2021, Pages 2084-2102.e19,
Madhav Mantri, Gaetano J. Scuderi, Roozbeh Abedini-Nassab, Michael F. Z. Wang, David McKellar, Hao Shi, Benjamin Grodner, Jonathan T. Butcher & Iwijn De Vlaminck	Spatiotemporal single-cell RNA sequencing of developing chicken hearts identifies interplay between cellular differentiation and morphogenesis	Nat Commun 12, 1771 (2021).
Kiryl D. Piatkevich, Seth Bensussen, Hua-an Tseng, Sanaya N. Shroff, Violeta Gisselle Lopez-Huerta, Demian Park, Erica E. Jung, Or A. Shemesh, Christoph Straub, Howard J. Gritton, Michael F. Romano, Emma Costa, Bernardo L. Sabatini, Zhanyan Fu, Edward S. Boyden & Xue Han	Volumetric live cell imaging with three-dimensional parallelized RESOLFT microscopy	Nat Biotechnol 39, 609–618 (2021).
Xinyu Wang, Chong Liu, Siwei Zhang, Huiwen Yan, Liwen Zhang, Amin Jiang, Yong Liu, Yun Feng, Di Li, Yuting Guo, Xinyao Hu, Yajing Lin, Pengcheng Bu, Dong Li,	N6-methyladenosine modification of MALAT1 promotes metastasis via reshaping nuclear speckles	Developmental Cell, Volume 56, Issue 5, 2021, Pages 702-715.e8,
Emily C. Wheeler, Anthony Q. Vu, Jaclyn M. Einstein, Matthew DiSalvo, Noorsher Ahmed, Eric L. Van Nostrand, Alexander A. Shishkin, Wenhao Jin, Nancy L. Allbritton & Gene W. Yeo	Pooled CRISPR screens with imaging on microraft arrays reveals stress granule-regulatory factors	Nature Methods volume 17, pages636–642 (2020)
Scott M. Emrich, Ryan E. Yoast, Ping Xin, Vikas Arige, Larry E. Wagner, Nadine Hempel, Donald L. Gill, James Sneyd, David I. Yule, Mohamed Trebak,	Omnitemporal choreographies of all five STIM/Orai and IP3Rs underlie the complexity of mammalian Ca2+ signaling	Cell Reports, Volume 34, Issue 9, 2021, 108760,
Tomomi Suzuki, Tetsuo Mioka, Kazuma Tanaka & Akira Nagatani	An optogenetic system to control membrane phospholipid asymmetry through flippase activation in budding yeast	Sci Rep 10, 12474 (2020).
Nicholas S. Groves,Merissa M. Bruns and Schuyler B. van Engelenburg	A Quantitative Live-Cell Superresolution Imaging Framework for Measuring the Mobility of Single Molecules at Sites of Virus Assembly	Pathogens 2020, 9(11), 972
Pierre Stömmer, Henrik Kiefer, Enzo Kopperger, Maximilian N. Honemann, Massimo Kube, Friedrich C. Simmel, Roland R. Netz & Hendrik Dietz	A synthetic tubular molecular transport system	Nat Commun 12, 4393 (2021).
Hugh Sparks, Lucas Dent, Chris Bakal, Axel Behrens, Guillaume Salbreux, and Chris Dunsby	Dual-view oblique plane microscopy (dOPM)	Biomed. Opt. Express 11, 7204-7220 (2020)
Philipp Zelger, Lisa Bodner, Martin Offterdinger, Lukas Velas, Gerhard J. Schütz, and Alexander Jesacher	Three-dimensional single molecule localization close to the coverslip: a comparison of methods exploiting supercritical angle fluorescence	Biomed. Opt. Express 12, 802-822 (2021)

PC recommendations

With the introduction of the ORCA-Fusion, users are now able to stream 5 megapixel images to their computers 89 frames per second. The computer recommendations for this high data rate can be met by using the guidelines listed this PC Recommendations for ORCA-Fusion.

PC Recommendation for ORCA-Fire / Quest2 / FusionBT / Fusion.pdf [588 KB/PDF]

Software

Our software provides the interface to access all of our carefully engineered camera features, from simply setting exposure to orchestrating complex triggering for multidimensional experiments.

Specifications

Type number	C14440-20UP
Imaging device	sCMOS
Effective no. of pixels	2304(H)×2304(V)
Cell size	6.5 μm(H)×6.5 μm(V)
Effective area	14.976 mm (H)×14.976 mm (V)
Full well capacity	15 000 electrons ^*1
Readout speed	Fast scan: 11.22 ms (89.1 frames/s with CoaXPress or 31.6 frames/s with USB 3.0) (2304 x 2304) ^5 Standard scan: 42.99 ms (23.2 frames/s with CoaXPress or USB 3.0) (2304 x 2304) ^5 Ultra quiet scan: 184.4 ms (5.4 frames/s with CoaXPress or USB 3.0) (2304 x 2304) ^*5
Readout noise	Fast scan：1.4 electrons rms ^1 Standard scan：1.0 electrons rms ^1 Ultra quiet scan：0.7 electrons rms ^*1
Exposure time	Fast scan: 17 μs to 10 s (4.87 μs step) Standard scan: 65 μs to 10 s (18.65 μs step) Ultra quiet scan: 280 μs\| 10 s (80.00 μs step)
Cooling method	Peltier cooling
Cooling temperature	Forced-air cooled: -5 ˚C (Ambient temperature: +25 ˚C) Water cooled: -5 ˚C (Water temperature: +25 ˚C) Water cooled (Max cooling): Less than -15 ˚C ^1^,3
Dark current	Cooling temperature(-5 ˚C): 0.5 electrons/pixel/s ^1^,4 Cooling temperature(-15 ˚C): 0.2 electrons/pixel/s ^1^,4
Dynamic range	21 400 : 1 ^1^,2
External trigger mode	Edge, Level, Sync readout, Start, Global reset edge, Global reset level
External trigger signal routing	SMA
Trigger delay function	0 s to 10 s in 1 μs steps
Trigger output	3 programmable timing, Global exposure timing, trigger ready, low, high
External signal output routing	SMA
Interface	CoaXPress (Dual CXP-6) and USB 3.0 ^*6
A/D converter	16 bit / 12 bit / 8 bit
Lens mount	C-mount
Power supply	Approx. 150 VA ^*7
Ambient operating temperature	0 ˚C to + 40 ˚C
Ambient operating humidity	30 % to 80 %, with no condensation

*1 Typical value
*2 Calculated from the ratio of the full well capacity and the readout noise
*3 The water temperature is +20 ˚C and the ambient temperature is +20 ˚C
*4 Dark current depends on cooling temperature
*5 Valid to 4 digits and rounded up to 5th digit
*6 USB 3.1 Gen 1 compatible
*7 The value with AC 240 V. (Approx. 70 VA with AC 100 V)