ORCA-Fusion Digital CMOS camera
The ORCA-Fusion, built from the sensor up, balances the complex nuances of camera features to provide beautiful images and robust data at all lights levels, but especially in tough low-light conditions. The exceptionally low and highly uniform read noise of the ORCA-Fusion means that when the sample emits even just a handful of photons, either by default or by experimental design, they are not lost in the noise, but detected and reliably quantified. After all, when you want to hear a whisper it’s best to be in a quiet place.
“Lightsheet Readout Mode” is a unique and patented feature of Hamamatsu sCMOS cameras which can improve signal to noise ratios in Lightsheet microscopy.
For more information about the principle and features of Lightsheet Readout Mode, please see details from below.
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Madhav Mantri, Gaetano J. Scuderi, Roozbeh Abedini-Nassab, Michael F. Z. Wang, David McKellar, Hao Shi, Benjamin Grodner, Jonathan T. Butcher & Iwijn De Vlaminck | Spatiotemporal single-cell RNA sequencing of developing chicken hearts identifies interplay between cellular differentiation and morphogenesis | Nat Commun 12, 1771 (2021). |
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Emily C. Wheeler, Anthony Q. Vu, Jaclyn M. Einstein, Matthew DiSalvo, Noorsher Ahmed, Eric L. Van Nostrand, Alexander A. Shishkin, Wenhao Jin, Nancy L. Allbritton & Gene W. Yeo | Pooled CRISPR screens with imaging on microraft arrays reveals stress granule-regulatory factors | Nature Methods volume 17, pages636–642 (2020) |
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With the introduction of the ORCA-Fusion, users are now able to stream 5 megapixel images to their computers 89 frames per second. The computer recommendations for this high data rate can be met by using the guidelines listed this PC Recommendations for ORCA-Fusion.
Our software provides the interface to access all of our carefully engineered camera features, from simply setting exposure to orchestrating complex triggering for multidimensional experiments.
Type number | C14440-20UP |
---|---|
Imaging device | sCMOS |
Effective no. of pixels | 2304(H)×2304(V) |
Cell size | 6.5 μm(H)×6.5 μm(V) |
Effective area | 14.976 mm (H)×14.976 mm (V) |
Full well capacity | 15 000 electrons *1 |
Readout speed | Fast scan: 11.22 ms (89.1 frames/s with CoaXPress or 31.6 frames/s with USB 3.0) (2304 x 2304) *5 Standard scan: 42.99 ms (23.2 frames/s with CoaXPress or USB 3.0) (2304 x 2304) *5 Ultra quiet scan: 184.4 ms (5.4 frames/s with CoaXPress or USB 3.0) (2304 x 2304) *5 |
Readout noise | Fast scan:1.4 electrons rms *1 Standard scan:1.0 electrons rms *1 Ultra quiet scan:0.7 electrons rms *1 |
Exposure time | Fast scan: 17 μs to 10 s (4.87 μs step) Standard scan: 65 μs to 10 s (18.65 μs step) Ultra quiet scan: 280 μs| 10 s (80.00 μs step) |
Cooling method | Peltier cooling |
Cooling temperature | Forced-air cooled: -5 ˚C (Ambient temperature: +25 ˚C) Water cooled: -5 ˚C (Water temperature: +25 ˚C) Water cooled (Max cooling): Less than -15 ˚C *1,*3 |
Dark current | Cooling temperature(-5 ˚C): 0.5 electrons/pixel/s *1,*4 Cooling temperature(-15 ˚C): 0.2 electrons/pixel/s *1,*4 |
Dynamic range | 21 400 : 1 *1,*2 |
External trigger mode | Edge, Level, Sync readout, Start, Global reset edge, Global reset level |
External trigger signal routing | SMA |
Trigger delay function | 0 s to 10 s in 1 μs steps |
Trigger output | 3 programmable timing, Global exposure timing, trigger ready, low, high |
External signal output routing | SMA |
Interface | CoaXPress (Dual CXP-6) and USB 3.0 *6 |
A/D converter | 16 bit / 12 bit / 8 bit |
Lens mount | C-mount |
Power supply | Approx. 150 VA *7 |
Ambient operating temperature | 0 ˚C to + 40 ˚C |
Ambient operating humidity | 30 % to 80 %, with no condensation |
*1 Typical value
*2 Calculated from the ratio of the full well capacity and the readout noise
*3 The water temperature is +20 ˚C and the ambient temperature is +20 ˚C
*4 Dark current depends on cooling temperature
*5 Valid to 4 digits and rounded up to 5th digit
*6 USB 3.1 Gen 1 compatible
*7 The value with AC 240 V. (Approx. 70 VA with AC 100 V)
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