ORCA-Flash4.0 LT3 Digital CMOS camera
The ORCA-Flash4.0 LT3 is a new scientific CMOS camera for fluorescence imaging, which has been improved from the ORCA-Flash 4.0 released in 2011.
It is equipped with a high level of performance required for fluorescence imaging such as life science applications, and can be used not only for basic research applications but also for integration into various types of equipment.
This site provides information on scientific cameras.
Since there is a wide range of camera types and performance, it is important to select the best camera for each application.
It introduces technical information, simulation tools, and examples of actual applications to help you fully understand the performance of the camera and select the best one for your application.
The graph below compares the readout noise distribution with the Gen II BSI camera* and shows that the ORCA-Flash4.0 LT3 has more pixels with smaller readout noise.
With a low readout noise of 1.5 electrons rms, the ORCA-Flash4.0 LT3 delivers high S/N comparable to or better than a BSI camera, especially in the low light range.
This performance can be demonstrated in high quality multidimensional imaging, as typified by long time-lapse imaging with low excitation light with low phototoxicity and photobleaching.
* BSI camera: Back-side illuminated camera
The ORCA-Flash4.0 LT3 is capable of 40 frames/second readout, which is even faster than the conventional ORCA-Flash4.0 LT+ (30 frames/second). This is useful for applications which requires real time measurement with high temporal resolution.
The wide field of view of 13.312 mm (H) × 13.312 mm (V) allows more information to be obtained in a single shot.
This contributes to higher throughput in experiments such as DNA chip reading.
With the introduction of the ORCA-Flash4.0 LT3, users are now able to stream 4 megapixel images to their computers 40 frames per second. The computer recommendations for this high data rate can be met by using the guidelines listed this PC Recommendations for ORCA-Flash4.0 LT3.
|Scientific CMOS image sensor
|Effective number of pixels
|2048 (H) × 2048 (V)
|6.5 μm (H) × 6.5 μm (V)
|13.312 mm (H) × 13.312 mm (V)
|Full well capacity (typ.)
|30 000 electrons
|Dynamic range (typ.)
|33 000: 1 *1 20 000: 1 *2
|Forced-air cooled, peltier cooling
|+10 ℃ (Forced-air cooled, FAN speed: Fast ambient temperature: +10 °C to +30 °C)
|Frame rate at full resolution
|Rapid rolling : 40 frames/s
Standard scan : 30 frames/s
|Readout noise (typ.)
|Rapid rolling : 1.9 electrons (rms) , 1.3 electrons (median)
Standard scan : 1.5 electrons rms / 0.9 electrons median
|Dark current (typ.)
|0.6 electrons/pixel/s (typ.) (Cooling temperature: +10 °C)
|Internal trigger mode *3 (Rapid rolling) : 1 ms to 10 s
Internal trigger mode *3 (Standard scan) : 3 ms to 10 s
Internal trigger mode with sub-array readout (Rapid rolling) : 40 μs to 10 s
Internal trigger mode with sub-array readout (Standard scan) : 129.99 μs to 10 s
|External trigger input mode
|Edge trigger, Global reset edge trigger, Level trigger, Global reset level trigger, Synchronous readout trigger, Start trigger
|Trigger delay function
|0 s to 10 s in 1 μs steps
|Trigger input connector
|3 programmable timing outputs, Trigger ready output, Global exposure timing output, Low output, High output
|Trigger output connector
|USB 3.1 Gen 1
|AC 100 V to AC 240 V, 50 Hz / 60 Hz
|Approx. 75 VA
|Ambient operating temperature
|0 °C to +40 °C (FAN speed: Fast)
|Ambient operating humidity
|30 % to 80 % (With no condensation)
|Ambient storage temperature
|−10 °C to +50 °C
|Ambient storage humidity
|90 % Max. (With no condensation)
*1 Calculated from the ratio of the full well capacity and the readout noise (median) in standard scan
*2 Calculated from the ratio of the full well capacity and the readout noise (rms) in standard scan
*3 Minimum exposure time in internal trigger mode varies depending on sub-array size and position.
It looks like you're in the . If this is not your location, please select the correct region or country below.
You're headed to Hamamatsu Photonics website for GB (English). If you want to view an other country's site, the optimized information will be provided by selecting options below.
For modern websites to work according to visitor’s expectations, they need to collect certain basic information about visitors. To do this, a site will create small text files which are placed on visitor’s devices (computer or mobile) - these files are known as cookies when you access a website. Cookies are used in order to make websites function and work efficiently. Cookies are uniquely assigned to each visitor and can only be read by a web server in the domain that issued the cookie to the visitor. Cookies cannot be used to run programs or deliver viruses to a visitor’s device.
Cookies do various jobs which make the visitor’s experience of the internet much smoother and more interactive. For instance, cookies are used to remember the visitor’s preferences on sites they visit often, to remember language preference and to help navigate between pages more efficiently. Much, though not all, of the data collected is anonymous, though some of it is designed to detect browsing patterns and approximate geographical location to improve the visitor experience.
Certain type of cookies may require the data subject’s consent before storing them on the computer.
This website uses two types of cookies:
There are two ways to manage cookie preferences.
If you wish to restrict or block web browser cookies which are set on your device then you can do this through your browser settings; the Help function within your browser should tell you how. Alternatively, you may wish to visit www.aboutcookies.org, which contains comprehensive information on how to do this on a wide variety of desktop browsers.
Occasionally, we may use internet tags (also known as action tags, single-pixel GIFs, clear GIFs, invisible GIFs and 1-by-1 GIFs) at this site and may deploy these tags/cookies through a third-party advertising partner or a web analytical service partner which may be located and store the respective information (including your IP-address) in a foreign country. These tags/cookies are placed on both online advertisements that bring users to this site and on different pages of this site. We use this technology to measure the visitors' responses to our sites and the effectiveness of our advertising campaigns (including how many times a page is opened and which information is consulted) as well as to evaluate your use of this website. The third-party partner or the web analytical service partner may be able to collect data about visitors to our and other sites because of these internet tags/cookies, may compose reports regarding the website’s activity for us and may provide further services which are related to the use of the website and the internet. They may provide such information to other parties if there is a legal requirement that they do so, or if they hire the other parties to process information on their behalf.
If you would like more information about web tags and cookies associated with on-line advertising or to opt-out of third-party collection of this information, please visit the Network Advertising Initiative website http://www.networkadvertising.org.
We use third-party cookies (such as Google Analytics) to track visitors on our website, to get reports about how visitors use the website and to inform, optimize and serve ads based on someone's past visits to our website.
You may opt-out of Google Analytics cookies by the websites provided by Google:
We inform you that in such case you will not be able to wholly use all functions of our website.