Biologists that use, or are interested in using, microscope cameras and don’t speak engineering
Clarity on the relevance of camera specs to biological experimentation
|Hamamatsu listing||Synonyms used by other vendors|
|Readout noise||Read noise|
Camera specs can give you an indication of whether or not the camera will be able to accurately image your sample, but image quality is dependent on the quality of your sample—as the commercials used to say, “actual results may vary,” and powerful, sensitive electronics cannot always makeup for high background or low contrast. With this caveat, the best indicators of camera sensitivity are a combination of quantum efficiency (QE) and camera noise.
QE is a measure of how many photons are converted to electrons by the sensor. QE is a function of the camera sensor materials and design and is dependent on the wavelength of the light being detected. In the camera specs, QE is presented as the percentage of photons converted into electrons—i.e. detected—at a particular wavelength, and manufacturers usually can provide QE versus wavelength plots.
Higher QEs generally indicate higher sensitivity, as the likelihood of detecting your signal is increased.
Camera noise is the amount of noise introduced by the camera. It primarily consists of two components—read noise and dark noise.
Dark current, and therefore dark noise, are temperature dependent, with less noise at lower temperatures. For most biological experiments, dark current and dark noise are negligible over a typical exposure interval of less than five minutes.
Because dark noise is typically negligible, the main noise component coming from the camera that needs to be considered is read noise.
It looks like you're in the . If this is not your location, please select the correct region or country below.
You're headed to Hamamatsu Photonics website for GB (English). If you want to view an other country's site, the optimized information will be provided by selecting options below.
For modern websites to work according to visitor’s expectations, they need to collect certain basic information about visitors. To do this, a site will create small text files which are placed on visitor’s devices (computer or mobile) - these files are known as cookies when you access a website. Cookies are used in order to make websites function and work efficiently. Cookies are uniquely assigned to each visitor and can only be read by a web server in the domain that issued the cookie to the visitor. Cookies cannot be used to run programs or deliver viruses to a visitor’s device.
Cookies do various jobs which make the visitor’s experience of the internet much smoother and more interactive. For instance, cookies are used to remember the visitor’s preferences on sites they visit often, to remember language preference and to help navigate between pages more efficiently. Much, though not all, of the data collected is anonymous, though some of it is designed to detect browsing patterns and approximate geographical location to improve the visitor experience.
Certain type of cookies may require the data subject’s consent before storing them on the computer.
This website uses two types of cookies:
There are two ways to manage cookie preferences.
If you wish to restrict or block web browser cookies which are set on your device then you can do this through your browser settings; the Help function within your browser should tell you how. Alternatively, you may wish to visit www.aboutcookies.org, which contains comprehensive information on how to do this on a wide variety of desktop browsers.
Occasionally, we may use internet tags (also known as action tags, single-pixel GIFs, clear GIFs, invisible GIFs and 1-by-1 GIFs) at this site and may deploy these tags/cookies through a third-party advertising partner or a web analytical service partner which may be located and store the respective information (including your IP-address) in a foreign country. These tags/cookies are placed on both online advertisements that bring users to this site and on different pages of this site. We use this technology to measure the visitors' responses to our sites and the effectiveness of our advertising campaigns (including how many times a page is opened and which information is consulted) as well as to evaluate your use of this website. The third-party partner or the web analytical service partner may be able to collect data about visitors to our and other sites because of these internet tags/cookies, may compose reports regarding the website’s activity for us and may provide further services which are related to the use of the website and the internet. They may provide such information to other parties if there is a legal requirement that they do so, or if they hire the other parties to process information on their behalf.
If you would like more information about web tags and cookies associated with on-line advertising or to opt-out of third-party collection of this information, please visit the Network Advertising Initiative website http://www.networkadvertising.org.
We use third-party cookies (such as Google Analytics) to track visitors on our website, to get reports about how visitors use the website and to inform, optimize and serve ads based on someone's past visits to our website.
You may opt-out of Google Analytics cookies by the websites provided by Google:
We inform you that in such case you will not be able to wholly use all functions of our website.