Japan (JA)
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2波長同時レシオイメージング
はじめに：レシオイメージングって何だろう？
蛍光ライブイメージングは生きた標本の活動をリアルタイムに観察出来る実験生物学の醍醐味と言える手法です。しかし、実際にこの手法で観察してみると「標本がどんどん暗くなり安定した結果が得られない」「高速で撮影すると輝度変化が小さくて計測ノイズなのか生体現象なのか区別がつかない」など、蛍光観察の安定性やデータの解釈についてお困りになった経験が有る方も多いのではないでしょうか。
このページでは上記の様な蛍光ライブイメージングに対して興味・あるいは課題をお持ちの方を対象に、レシオイメージングと呼ばれる蛍光計測手法についてその特長と可能性を解説します。レシオイメージングは、1つの蛍光プローブからの2つの波長の蛍光を計測する手法です。1つの波長の蛍光観察でも済むことを、わざわざ2つの波長を用いて計測する、何だか面倒な計測だと思われる方もいるかもしれません。しかし、レシオイメージングは生命現象に由来する微小な蛍光輝度変化を、より正確に計測する上で多くのメリットを持っています。まず、このメリットを模式的な計測例を用いて説明します。 次に従来手法では捉えることが困難だった心筋細胞の膜電位変化のような高速な生命現象を2波長同時レシオイメージングのメリットを活かして捉える方法について、実測例を交えながら紹介します。
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This website uses two types of cookies:
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3. How do we use cookies?
This website uses cookies for following purposes:
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Cookies help us help you. Through the use of cookies, we learn what is important to our visitors and we develop and enhance website content and functionality to support your experience. Much of our website can be accessed if cookies are disabled, however certain website functions may not work. And, we believe your current and future visits will be enhanced if cookies are enabled.
4. Which cookies do we use?
There are two ways to manage cookie preferences.
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Occasionally, we may use internet tags (also known as action tags, single-pixel GIFs, clear GIFs, invisible GIFs and 1-by-1 GIFs) at this site and may deploy these tags/cookies through a third-party advertising partner or a web analytical service partner which may be located and store the respective information (including your IP-address) in a foreign country. These tags/cookies are placed on both online advertisements that bring users to this site and on different pages of this site. We use this technology to measure the visitors' responses to our sites and the effectiveness of our advertising campaigns (including how many times a page is opened and which information is consulted) as well as to evaluate your use of this website. The third-party partner or the web analytical service partner may be able to collect data about visitors to our and other sites because of these internet tags/cookies, may compose reports regarding the website’s activity for us and may provide further services which are related to the use of the website and the internet. They may provide such information to other parties if there is a legal requirement that they do so, or if they hire the other parties to process information on their behalf.
If you would like more information about web tags and cookies associated with on-line advertising or to opt-out of third-party collection of this information, please visit the Network Advertising Initiative website http://www.networkadvertising.org.
6. Analytics and Advertisement Cookies
We use third-party cookies (such as Google Analytics) to track visitors on our website, to get reports about how visitors use the website and to inform, optimize and serve ads based on someone's past visits to our website.
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As provided in this Privacy Policy (Article 5), you can learn more about opt-out cookies by the website provided by Network Advertising Initiative:
http://www.networkadvertising.org
We inform you that in such case you will not be able to wholly use all functions of our website.
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