The method of live-cell single-molecule fluorescence imaging plays an important role on various life science research fields, and sensitivity and resolution of the detector is critical for the imaging performance.
In order to achieve the best performance of single molecule imaging, Dr. Jiachao Xu, from the Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, built a TIRF microscope and has been continuously improving it in past several years.
Recently, Dr. Xu compared the performance of two kinds of cameras, EM-CCD and qCMOS, in his experiment of single molecule fluorescence imaging of angiotensin II type 1 receptor (AT1R) in living cells.
Dr. Jiachao Xu’s experiment requires the detector comes with very low noise, so as the fluorescence of single molecules to be easily recognized from a relatively clean background.
In his experience, EM-CCD could offer a good signal to noise ratio. He also tried some other types of cameras before, but none could achieve similar performance.
But this time qCMOS shows excellent performance with a readout noise almost as low as EM-CCD’s, even at the fast mode (0.43e r.m.s.). Almost the same quantity of fluorescent single molecules could be seen from the image of qCMOS and EM-CCD.
In addition, qCMOS has a smaller pixel size of 4.6 ㎛, which means it could be used under some modes: 4.6 ㎛ (1×1 binning), 9.2 ㎛ (2×2 binning) and 18.4 ㎛ (4×4 binning) depending on imaging demands. For example, to balance between sensitivity and localization precision, Dr. Xu choosed 9.2 ㎛ (2×2 binning) setting for the best performance.
In summary, qCMOS shows comparable or even better performance compared with EM-CCD on single-molecule fluorescence imaging. Its advantages on sensitivity, speed and resolution could provide more choices for different scientific imaging applications.
Exposure time：100 ms
Objective lens : 100× NA 1.49
laser：488 nm 1 mW
Dr. Jiachao Xu is from Dr. Kangmin He’s laboratory. Their research is highly praised in the field. The research method of real-time live-cell single particle tracking using TIRFM is "exciting, which will promote many peers to carry out similar research".
It looks like you're in the . If this is not your location, please select the correct region or country below.
You're headed to Hamamatsu Photonics website for US (English). If you want to view an other country's site, the optimized information will be provided by selecting options below.
For modern websites to work according to visitor’s expectations, they need to collect certain basic information about visitors. To do this, a site will create small text files which are placed on visitor’s devices (computer or mobile) - these files are known as cookies when you access a website. Cookies are used in order to make websites function and work efficiently. Cookies are uniquely assigned to each visitor and can only be read by a web server in the domain that issued the cookie to the visitor. Cookies cannot be used to run programs or deliver viruses to a visitor’s device.
Cookies do various jobs which make the visitor’s experience of the internet much smoother and more interactive. For instance, cookies are used to remember the visitor’s preferences on sites they visit often, to remember language preference and to help navigate between pages more efficiently. Much, though not all, of the data collected is anonymous, though some of it is designed to detect browsing patterns and approximate geographical location to improve the visitor experience.
Certain type of cookies may require the data subject’s consent before storing them on the computer.
This website uses two types of cookies:
There are two ways to manage cookie preferences.
If you wish to restrict or block web browser cookies which are set on your device then you can do this through your browser settings; the Help function within your browser should tell you how. Alternatively, you may wish to visit www.aboutcookies.org, which contains comprehensive information on how to do this on a wide variety of desktop browsers.
Occasionally, we may use internet tags (also known as action tags, single-pixel GIFs, clear GIFs, invisible GIFs and 1-by-1 GIFs) at this site and may deploy these tags/cookies through a third-party advertising partner or a web analytical service partner which may be located and store the respective information (including your IP-address) in a foreign country. These tags/cookies are placed on both online advertisements that bring users to this site and on different pages of this site. We use this technology to measure the visitors' responses to our sites and the effectiveness of our advertising campaigns (including how many times a page is opened and which information is consulted) as well as to evaluate your use of this website. The third-party partner or the web analytical service partner may be able to collect data about visitors to our and other sites because of these internet tags/cookies, may compose reports regarding the website’s activity for us and may provide further services which are related to the use of the website and the internet. They may provide such information to other parties if there is a legal requirement that they do so, or if they hire the other parties to process information on their behalf.
If you would like more information about web tags and cookies associated with on-line advertising or to opt-out of third-party collection of this information, please visit the Network Advertising Initiative website http://www.networkadvertising.org.
We use third-party cookies (such as Google Analytics) to track visitors on our website, to get reports about how visitors use the website and to inform, optimize and serve ads based on someone's past visits to our website.
You may opt-out of Google Analytics cookies by the websites provided by Google:
We inform you that in such case you will not be able to wholly use all functions of our website.