Biologists whose physics is a little rusty.
A quick review of dark noise
Dark noise is statistical variation in the number of electrons thermally generated within the pixel in a photon-independent fashion, and is the electron equivalent of photon shot noise. Dark noise is calculated from the dark current:
Dark current, and therefore dark noise, are temperature dependent, with less noise at lower temperatures. For most biological experiments, dark current and dark noise are negligible over a typical exposure interval of less than five minutes.
Because dark noise is typically negligible, the main noise component coming from the camera that needs to be considered is read noise.
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