ORCA-Lightning Digital CMOS camera
For imaging scientists, inspiration is often found at the edges―at the spatial, temporal, and optical boundaries of a system. It’s that grainy image of the cytoskeleton that has just enough photons to confidently resolve two unique molecules. It’s data extracted from a few pixels, whittled away from a larger array, to achieve capture rates relevant to neuronal signaling. It’s the mesmerizing cellular migrations of a developing embryo, reconstructed from a lightsheet time-lapse, that offer new insights and spark new questions.
By more than doubling speed, resolution and field of view relative to current scientific cameras, the ORCA-Lightning is designed to inspire new frontiers of exploration. Compared to a Gen II sCMOS, the ORCA-Lightning delivers 2 times the pixel area, 2.8 times the pixels and 3.4 times faster pixel-per-second readout.
All these increases translate to the ability to capture more image data faster, and still achieve Hamamatsu-quality images and information content. So whether you’re developing the next cutting-edge lightsheet system or building a library of the mouse connectome, the high throughput of the ORCA-Lightning will get you to your goal faster. And, we hope, open doors to new discoveries.
Can even take high-speed images which were difficult to fully capture until now, with approximately 6 times the readout speed, while maintaining a level of readout noise similar to that of ORCA-Flash4.0 V3.
Speed comparison with ORCA-Flash4.0 V3
Cameras used in light-sheet microscopes put emphasis on high speed, demanding fast readout. Because images with increased readout speed result in obtaining fewer photons upon capture, it is necessary to keep the camera's readout noise low when trying to capture high S/N images. ORCA-Lightning can capture images at approximately 6 times the readout speed, while maintaining a level of readout noise similar to that of ORCA-Flash4.0 V3.
Using CoaXPress as an interface and optimizing high speed data transfer to the PC, ORCA-Lightning realizes high-speed image acquisition.
Customers can select the best mode depending on their usage.
In addition to a readout from a Standard Full Well Capacity mode (12 bit), ORCA-Lightning also supports a readout from a High Full Well Capacity mode (16 bit) for high dynamic range.
|Effective number of pixels
(H) × (V)
|Readout speed (frames per second)
|Free running mode *1
|Edge trigger mode *2
|Synchronous readout trigger mode *3
|Global reset trigger mode *4
|4608 × 2592
|2688 × 2592
|2560 × 2560
|2048 × 2048
|1024 × 1024
|512 × 512
|256 × 256
|128 × 4
*1 Free running mode is operated via camera alone.
*2 Edge trigger mode starts the exposure by the edge (rising/falling edge) of the trigger signal input to the external device.
*3 Synchronous readout trigger mode ends the exposure of the camera and readout is started, while the next exposure is simultaneously started by the edge (rising/falling edge) of the trigger signal input to the external device.
*4 Global reset trigger mode starts the exposure of all pixels all together by synchronizing the trigger signal from external device.
We took consecutive images and produced 3D imaging of a free-swimming Volvox.
When capturing the image of a free-swimming Volvox, imaging speed of 2-dimensional (area of observation) and 3-dimensional (number of images) settings were selected with a precondition of approximately 8 volumes per second.
With its high speed, ORCA-Lightning can capture clearer images within a wide range compared to ORCA-Flash4.0 V3.
Collaborator: Laboratory for Spatiotemporal Regulations Exploratory Research Center on Life and Living Systems (ExCELLS) / National Institute for Basic Biology (NIBB), Dr.Atsushi Taniguchi, Dr.Shigenori Nonaka
Comparison of sensor size between ORCA-Flash4.0 V3 and ORCA-Lightning
ORCA-Lightning covers the twice large field of view compared to ORCA-Flash4.0 V3, so that it can improve throughput and efficiency of work.
Collaborator: Department of Cellular Neurobiology Graduate School of Medicine the University of Tokyo, Dr.Yutaro Kashiwagi, Dr.Shigeo Okabe
Collaborator: Laboratory for Spatiotemporal Regulations Exploratory Research Center on Life and Living Systems (ExCELLS) / National Institute for Basic Biology (NIBB), Dr. Atsushi Taniguchi, Dr. Shigenori Nonaka
Sample provider: National Institute for Basic Biology (NIBB), Spectrography and BioImaging Facility, Joe Sakamoto Ph. D (NBRP Medaka)
With the introduction of the ORCA-Lightning, users are now able to stream 12 megapixel images to their computers 121 frames per second. The computer recommendations for this high data rate can be met by using the guidelines listed this PC Recommendations for ORCA-Lightning.
|Scientific CMOS image sensor
|Effective no. of pixels
|4608 (H) × 2592 (V)
|5.5 μm (H) × 5.5 μm (V)
|25.344 mm (H) × 14.256 mm (V)
|Full well capacity
|Standard Full Well Capacity mode: 1000 electrons
High Full Well Capacity mode: 38 000 electrons
|121 frames/s (at full resolution)
|Standard Full Well Capacity mode：1.5 electrons median
Standard Full Well Capacity mode：2.0 electrons rms
High Full Well Capacity mode：2.2 electrons median
High Full Well Capacity mode: 2.7 electrons rms
|Standard Full Well Capacity mode: 6.304 μs to 1 s
High Full Well Capacity mode: 50.432 μs to 1 s
|Forced-air cooled (Ambient temperature: +25 ˚C): +20 ˚C
Water cooled: +20 ˚C
|15 electrons/pixel/s (Typ.) : Air cooled (cooling temperature: +20 ˚C)
|Standard Full Well Capacity mode: 650 : 1
High Full Well Capacity mode: 17 000 : 1
|External trigger input
|Edge trigger, Global reset edge trigger, Level trigger, Global reset level trigger, Synchronous readout trigger, Start trigger
|External trigger signal routing
|Trigger delay function
|0 s to 10 s in 1 μs steps
|CoaXPress (Quad CXP-6)
|Standard Full Well Capacity mode: 12 bitHigh Full Well Capacity mode: 16 bit
|Approx. 200 VA
|Ambient operating temperature
|0 ˚C to +40 ˚C : Forced-air cooled (With no condensation)
|Ambient operating humidity
|30 % to 80 %
It looks like you're in the . If this is not your location, please select the correct region or country below.
You're headed to Hamamatsu Photonics website for GB (English). If you want to view an other country's site, the optimized information will be provided by selecting options below.
For modern websites to work according to visitor’s expectations, they need to collect certain basic information about visitors. To do this, a site will create small text files which are placed on visitor’s devices (computer or mobile) - these files are known as cookies when you access a website. Cookies are used in order to make websites function and work efficiently. Cookies are uniquely assigned to each visitor and can only be read by a web server in the domain that issued the cookie to the visitor. Cookies cannot be used to run programs or deliver viruses to a visitor’s device.
Cookies do various jobs which make the visitor’s experience of the internet much smoother and more interactive. For instance, cookies are used to remember the visitor’s preferences on sites they visit often, to remember language preference and to help navigate between pages more efficiently. Much, though not all, of the data collected is anonymous, though some of it is designed to detect browsing patterns and approximate geographical location to improve the visitor experience.
Certain type of cookies may require the data subject’s consent before storing them on the computer.
This website uses two types of cookies:
There are two ways to manage cookie preferences.
If you wish to restrict or block web browser cookies which are set on your device then you can do this through your browser settings; the Help function within your browser should tell you how. Alternatively, you may wish to visit www.aboutcookies.org, which contains comprehensive information on how to do this on a wide variety of desktop browsers.
Occasionally, we may use internet tags (also known as action tags, single-pixel GIFs, clear GIFs, invisible GIFs and 1-by-1 GIFs) at this site and may deploy these tags/cookies through a third-party advertising partner or a web analytical service partner which may be located and store the respective information (including your IP-address) in a foreign country. These tags/cookies are placed on both online advertisements that bring users to this site and on different pages of this site. We use this technology to measure the visitors' responses to our sites and the effectiveness of our advertising campaigns (including how many times a page is opened and which information is consulted) as well as to evaluate your use of this website. The third-party partner or the web analytical service partner may be able to collect data about visitors to our and other sites because of these internet tags/cookies, may compose reports regarding the website’s activity for us and may provide further services which are related to the use of the website and the internet. They may provide such information to other parties if there is a legal requirement that they do so, or if they hire the other parties to process information on their behalf.
If you would like more information about web tags and cookies associated with on-line advertising or to opt-out of third-party collection of this information, please visit the Network Advertising Initiative website http://www.networkadvertising.org.
We use third-party cookies (such as Google Analytics) to track visitors on our website, to get reports about how visitors use the website and to inform, optimize and serve ads based on someone's past visits to our website.
You may opt-out of Google Analytics cookies by the websites provided by Google:
We inform you that in such case you will not be able to wholly use all functions of our website.