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Technical guides

Concise articles about camera technology and its use

Relative SNR—How does your camera compare?

Compare microscope cameras or understand how specs affect performance.

How many photons do I have?

Find out how to measure how many photons are present under your experimental conditions.

Calculating SNR

Understand the different components that go into calculating the signal-to-noise ratio (SNR).

A visual guide to CCD vs. EM-CCD vs. CMOS

Different chip architectures, different capabilities.

Contrast versus noise, eye versus machine.

Contrast appears good, but good looks can be deceiving.

What is dark noise?

A basic introduction.

Dissecting camera specifications: a field guide for biologists

Shedding light on what camera specifications mean for biological experiments.

What is photon shot noise?

A basic introduction.

Read noise in CMOS cameras: only rms is meaningful

A brief overview of median versus rms read noise for evaluating CMOS cameras.

Thinking in Photons.

Converting from electrons to photons improves how we think about signal-to-noise ratio (SNR).

Which camera technology is right for me?

Choosing between CCD, EM-CCD, and sCMOS.

It's all about noise and how you deal with it.

An overview of why Huang, et al, (2013), generated new software algorithms for precision localization (using SMSN) with an sCMOS camera.

Can You Trust What You See?

A short guide to reliable microscopy imaging.

Capturing images you can count on.

A checklist for microscopy imaging.

 
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