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ImagEM X2 EM-CCD camera C9100-23B

c9100-23b product photo
Catalog [557 KB/PDF]

The ImagEM X2 is an extremely versatile camera that quietly delivers 70 frames/s at full frame and up to 1076 frames/s with analog binning and regions of interest. With very high signal to noise in near dark conditions and extremely low dark current, the ImagEM X2 enables quantitative ultra-low light imaging both for long integration times and at high speed. With EM gain off, the extremely deep full well capacity can extract information from the lowest contrast bright images. Additional new features allow for optimized camera triggering, on-board for EM gain protection, streamlined connectivity through IEEE1394b, improved overall signal to noise and increased non-EM dynamic range. Hamamatsu has taken the beloved 512 × 512 EM-CCD sensor and created a masterfully redesigned camera that delivers maximum speed and precision performance.
The ImagEM X2-1K(C9100-24B) 1024×1024 version is also available.

Hungry for Photons

With large pixels, high QE, and relatively zero readout noise, EM-CCD technology performs in low light conditions. How low light? When you've got fewer than 10 photons per pixel between the sample and background, EM-CCDs are the perfect tool for the job, delivering the best SNR of any camera technology. For high mag, biologically relevant applications with routine exposure times of 10 ms to 30 ms, the sample is likely emitting hundreds to thousands of photons per pixel. But with faster speeds come shorter exposure times, risking the ability to capture more than tens of photons per pixel in one shot and therefore pushing the application into the ultra-low light zone. The ImagEM X2 makes these super-fast exposures possible and has the sensitivity to provide visually pleasing and quantitatively meaningful images in a photon-starved environment.

New Features

Faster readout

By clocking pixel readout at 22 MHz, the ImagEM X2 is able to achieve 70 frames/s with full frame resolution. That's more than 2× the original ImagEM and is faster than any commercially available camera using the sensor.

Clock: 22 MHz

Binning Effective vertical width (Sub-array)
512 256 128 64 32 16
1×1 70.4 133 241 405 613 820
2×2 131 238 400 606 813 981
4×4 231 389 588 794 962 1076

(Unit: frames/s)

Corner readout

By selectively imaging at the edge of the sensor, closest to the read register of the chip, it is possible to achieve even greater speeds of small ROIs.

Clock: 22 MHz

Binning Effective vertical width (Sub-array)
512 256 128 64 32 16
1×1 70.4 133 285 495 741 893
2×2 131 238 456 699 901 981
4×4 231 389 645 863 981 1076

(Unit: frames/s)

Lower readout noise

In any image sensor, faster readout means increased readout noise. Yet readout noise is considered irrelevant for EM-CCDs because of the EM gain. Remarkably, the ImagEM X2, even before applying EM gain, has faster speed and lower read noise than its predecessor. But didn’t we just say readout noise in EM-CCD was irrelevant? Yes, in SNR equations this is true. However, if the primary purpose of EM gain is to overcome readout noise, then this will be accomplished with less gain in the ImagEM X2 and less voltage in the EM register, translating into theoretically more stable EM gain calibrations and greater sensor longevity.

Readout noise (rms) (typ.) EM-CCD readout EM gain 4× 36 electrons (at 22 MHz)
25 electrons (at 11 MHz)
8 electrons (at 0.6875 MHz)
EM gain 1200× 1 electron max. (at 22 MHz)
1 electron max. (at 11 MHz)
1 electron max. (at 0.6875 MHz)
NORMAL CCD readout 8 electrons (at 0.6875 MHz)

Mechanical shutter

The ImagEM X2 includes an integrated mechanical shutter in order to protect the camera from EM gain degradation and to lessen afterimage effects. The mechanical shutter is software controlled.

EM gain measurement and calibration

Gain aging is a known and expected process in EM technology. Even when every care is taken to minimize gain aging, use of the camera in EM mode, especially with high gains or high intensity light, can degrade the gain. Since this is a use-dependent phenomenon, it's important to know when it's happened and to have the ability to easily recalibrate. These two functions in the ImagEM X2 make this crucial maintenance of the camera software accessible and user friendly.

IEEE 1394b connectivity

The data rates of the ImagEM X2 are well suited to the trusted and easy to use 1394b connectivity.

SMA triggering ports

In its new incarnation, the ImagEM X2 sports four shiny and compact SMA ports, one for input of an external trigger and three for output to other devices. These ports can be used to access an array of triggering options including three additional features: programmable trigger input/output, trigger delay and trigger ready. There is no denying that EM-CCD technology offers the best SNR for ultra-low light imaging, and the ImagEM X2 offers the fastest speeds combined with multiple engineering enhancements to allow you to make the most of this technology.

Direct electron display

Output signal can be indicated in "electrons" instead of pixel AD counts in application software.

Black Clip / White Clip function

It enables setting an upper or lower threshold of intensity. If there is a brighter or darker location than a sample of interest in an image, this function allows clipping the upper limit or lower limit of intensity to make Auto LUT function work effectively.

Cooling status output

The camera indicates when it has reached the target cooling temperature.

 

Features

High Sensitivity

High QE

c9100-23b product feature

High EM gain of maximum 1200×

EM gain feature is ideal for live cell imaging because of shorter exposure times and reduced excitation light levels.

c9100-23b product feature

Low Noise

Minimal dark noise is another benefit of stable cooling performance

The dark current of a CCD depends on the temperature, and it decreases by half when the temperature drops by approximately 7 to 8 °C. Therefore, cooling a CCD is a very good way to reduce dark current noise. The ImagEM X2's stable cooling enables stable output and its water cooling minimizes dark current.

Highly stabilized control of sensor temperature with either water or forced-air cooling

Water or forced-air cooling is selectable for any application, and optimal cooling temperature can be set in each cooling mode.

Optimized sensor drive methods significantly reduce the clock induced charge (CIC)

Dark current consists of thermal charge and clock induced charge (CIC). CIC will dominate the dark charge in the images taken at short exposure time, and thermal charge will dominate the dark charge in images taken at longer exposures. The camera is adjusted to use the optimized drive method suitable to the scan speed. The biologist doesn't have to think about CIC optimization for long or short exposures. The camera handles it automatically.

c9100-23b product feature

Great Stability

Highly stabilized EM gain by cooling temperature control

Maintaining stable cooling temperature is essential to stable gain settings required for superior performance in long duration imaging and analysis. Very precise control of the cooling temperature in the ImagEM X2 is a key benefit.

c9100-23b product feature

Stability of mean bias value (Digitizer Offset)

The baseline is constant over time providing signal stability for long term measurements.

c9100-23b product feature

EM gain protection

It is important to operate the camera in ways that minimize the rate of gain aging and extend the life of the camera. The ImagEM X2 protects EM gain in two levels: EM gain warning and EM gain protection. EM gain protection mode stops charge transfer through the EM gain register when excessive output conditions have occurred which may damage the sensor.

EM gain readjustment *

Over time all EM-CCD cameras exhibit gain degradation. The EM gain can be readjusted by raising the voltage in the multiplying register. The EM gain readjustment can be done by software which comes with the camera. However, the number of times the EM gain can be readjusted is limited.

*This feature is available when the camera is operated with DCAM-API. (DCAM-API is a software driver which supports HAMAMATSU digital cameras.)

Selectable Readout Modes

Select a readout mode for optimal image acquisition based on the sample brightness or desired frame rate or exposure time.

c9100-23b sample image

An often overlooked benefit of EM-CCD technology is the ability to utilize the camera as a standard CCD. In non-EM mode, there is no effect of excess noise, and the large full well capacity and high dynamic range are ideal for bright light applications that have large intrascene dynamic range. The ImagEM X2 provides a low read noise non-EM mode that can be an ideal choice for such applications.

Photon Imaging Mode

This is a unique technology to improve image quality at very low light level to overcome the limitation of excess noise factor from the electron multiplying process. This mode is most useful for signal levels at which maximum EM gain has no apparent signal or very little signal. The mode preserves quantitative linear signal output and also improves spatial resolution at very low light levels.

c9100-23b sample image

On-board Image Processing

The following real time processing functions are available.

  • Background subtraction
    Effective for reducing fluorescence in image backgrounds.
  • Shading correction
    This feature corrects the shading or uneven illumination in microscope images or other illumination systems.
  • Recursive filter
    This feature provides random noise elimination in an image by weighted time based averaging.
  • Frame averaging
    This feature provides noise elimination in an image by simple frame averaging and less “afterimage” effect than the recursive filter.
  • Spot noise reducer
    This image processing function operates on random spots of intensity by comparing incoming images and eliminating signals that meet the criteria for noise in one image but not in others. This processing eliminates noise elements like cosmic rays.

Applications

  • Protein-protein interaction
  • Calcium waves in cell networks and intracellular ion flux
  • Real time spinning disk confocal microscopy
  • Single molecule imaging with TIRF microscopy
  • Fluorescence in-vivo blood cell microscopy
  • Gene expression imaging using luminescence
c9100-23b application example
 

Configuration

c9100-23b system configuration

Specifications

Type number C9100-23B
Camera head type Hermetic vacuum-sealed air/water-cooled head*1
Window Anti-reflection (AR) coatings on both sides, single window
AR mask Yes
Imaging device Electron Multiplying Back-Thinned Frame Transfer CCD
Effective number of pixels 512 (H)×512 (V)
Cell size 16 μm (H)×16 μm (V)
Effective area 8.19 mm (H) × 8.19 mm (V)
Pixel clock rate (EM-CCD readout) 22 MHz, 11 MHz, 0.6875 MHz
Pixel clock rate (NORMAL CCD readout) 0.6875 MHz
EM (electron multiplying) gain 4× to 1200× (typ.)*2
Ultra low light detection Photon Imaging mode (1, 2, 3)
Fastest readout speed 70.4 frames/s to 1076 frames/s
Readout noise (EM-CCD readout) 36 electrons (EM gain 4×, at 22 MHz) (rms) (typ.)
25 electrons (EM gain 4×, at 11 MHz) (rms) (typ.)
8 electrons (EM gain 4×, at 0.6875 MHz) (rms) (typ.)
1 electron max. (EM gain 1200×) (rms) (typ.)
Readout noise (NORMAL CCD readout) 8 electrons (at 0.6875 MHz) (rms) (typ.)
Full well capacity EM-CCD readout: 370 000 electrons (Max. 800 000 electrons) (typ.)*3
NORMAL CCD readout: 140 000 electrons (typ.)
Analog gain EM-CCD readout (22 MHz): 1×
EM-CCD readout (11 MHz/0.6875 MHz): 0.5×, 1×
Normal CCD readout: 1×, 2×, 3×, 4×, 5×
Cooling temperature (Forced-air cooled) At temperature control: -65 ℃ stabilized (0 ℃ to +30 ℃)
At maximum cooling (typ.): -80 ℃ (Room temperature: stable at +20 ℃)
Cooling temperature (Water cooled) At temperature control: -80 ℃ stabilized (Water temperature: +20 ℃)
At maximum cooling (typ.): -100 ℃ (Water temperature: lower than +10 ℃)
Temperature stability (Forced-air cooled) ±0.01 ℃ (typ.)
Temperature stability (Water cooled) ±0.01 ℃ (typ.)
Dark current (Forced-air cooled) 0.005 electron/pixel/s (-65 ℃) (typ.)
Dark current (Water cooled) 0.0005 electron/pixel/s (-80 ℃) (typ.)
Clock induced charge 0.0015 events/pixel/frame (typ.)
Exposure time (Internal sync mode) 13.9 ms to 1 s (22 MHz)
27.2 ms to 2 h (11 MHz)
421.5 ms to 2 h (0.6875 MHz)
Exposure time (External trigger mode) 10 μs to 1 s (22 MHz)
10 μs to 2 h (11 MHz, 0.6875 MHz)
A/D converter 16 bit
Output signal/External control IEEE1394b
Sub-array Every 16 lines (horizontal, vertical) size, position can be set
Binning 2×2, 4×4, 8×8, 16×16*4
External trigger mode Edge trigger, Level trigger, Start trigger, Synchronous readout trigger
Trigger output Exposure timing output, Programmable timing output (Delay and pulse length are variable.), Trigger ready output
Image processing features (real-time) Background subtraction, Shading correction, Recursive filter, Frame averaging, Spot noise reducer*5
EM gain protection EM warning mode, EM protection mode
EM gain readjustment Available
Lens mount C-mount
Power supply AC 100 V to 240 V, 50 Hz / 60 Hz
Power consumption Approx. 140 VA
Ambient storage temperature -10 °C to + 50 °C
Ambient operating temperature 0 °C to + 40 °C
Performance guaranteed temperature 0 °C to + 30 °C
Ambient operating/storage humidity 70 % max. (with no condensation)

*1 The hermetic sealed head maintains a high degree of vacuum, 10-8 Torr, without re-evacuation.
*2 Even with electron multiplying gain maximum, dark signal is kept at a low level during low light imaging.
*3 Linearity is not assured when full well capacity is over 370 000 electrons, because of CCD performance.
*4 8×8 and 16×16 binning are available on special order.
*5 Recursive filter, frame averaging, and spot noise reducer cannot be used simultaneously.

Spectral response

c9100-23b spectral response

Dimensions

c9100-23b dimensional outline

Publications

Publications list

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